EFFECTS OF LIGHT ILLUMINATION AND SUBCULTURE FREQUENCY ON BIOMASS PRODUCTION IN CELL SUSPENSION CULTURES OF Clinacanthus nutans

Clinacanthus nutans (Burm. f.) Lindau is a medicinal plant rich in polyphenols and possesses multiple pharmacological properties. It widely used as side treatment for cancer to prevent oxidative stress-related diseases. The present study aims investigate the effects of light illumination subculture frequency on growth proliferation cells suspension culture C. nutans. Friable callus induced from leaf explants were cultured liquid Murashige & Skoog medium supplemented with 0.25 mg/L 2, 4-dichlorophenoxyacetic acid, 6-benzylaminopurine initiate cell culture. cultures incubated under different conditions (16 hr photoperiod continuous darkness). Subcultures conducted every two weeks ten cycles. Results revealed that higher biomass was obtained exposure 16 highest production at fifth cycle (growth index 11.8) exhibiting stable eighth tenth cycle. showed rates up 10th producing small aggregates single cells. indicated significantly impacts valuable secondary metabolites.